|
KMID : 0545120030130060976
|
|
Journal of Microbiology and Biotechnology
2003 Volume.13 No. 6 p.976 ~ p.983
|
|
|
Identification of Genes for Growth with Oxygen in Escherichia coli by Operon Fusion and Southern Blot Techniques
|
|
KIM, IL-MAN
LEE, YONGCHAN/WON, JAESEON/CHOE, MUHYEON
|
|
|
Abstract
|
|
|
Seven Escherichiu coli cells defective with aerobic growth were isolated by the insertion of hplacMuS3, a hybrid bacteriophage of h and Mu, which created a transcriptional fusion to 1acZY These insertion mutant cells were tested on an XG (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside) medium for anaerobic expression of Iac2 by fusion to a promoter. The chromosomal DNA from these strains were digested by EcoRI, and the EcoRI fragments that contained the fused gene and IucZ sequence were identified by Southern hybridization, using lacZ containing plasmid as a probe. The EcoRI fragment from each strain was cloned and sequenced. The sequence data were compared with the GenBank database. The mutated gene of three strains, CYT4. CYTS, and Os1I , was found to be identical, and it was nrdAB that encoded ribonucleoside diphosphate reductase. The gene nrriAB was at min 50.5 on the Escherichiu coli linkage map and 2,348,084 on the physical map, and is involved in heme-related reduction-oxidation reaction. OS6 and 0s 14 mutant strains had insertion at min 8.3 and the mutated gene was hemB. The hemB encodes 5-aminolevulinate dehydratase or porphobilinogen synthase.
The OS3 mutant had insertion in cydB at min 16.6. The cydAB encodes cytochrome d oxidase. In the case of OS1, the fusion was made with mcA, the El component of α-ketoglutarate dehydrogenase.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|